Contact an Account ManagerSlow isomerization of some proline-containing peptides inducing peak splitting during reversed phase HPLC
Summary
Type: HPLC
Application #: 107860
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 215nm.
Conditions: eluent: A, phosphate buffer pH 3.5 containing 3% acetonitrile, B, phosphate buffer pH 3.5 containing 50% acetonitrile, gradient from 100% A to 100% B in 15min
flow rate 0.7ml/min
Authors: Gesquiere JC
Source: J Chromatogr 478 (1989) 121 - 129
Details
Legend: Fig. 1. HPLC of peptide I (Met-Ser-Ile-Pro-Pro-Glu-Lys) as a function of temperature.